Figure 6.

LS-1-10 reduces cancer cell viability. (a) DLD1 and LoVo cells were treated with varying concentrations of LS-1-10 or chloroquine (CQ) as indicated for 72 h. Cell viability was measured by CCK-8 assay. Data represent the means±S.D. (n=3). (b) DLD1 and LoVo cells were treated with LS-1-10 or amsacrine (chemical structures shown in the left panel) at the indicated concentrations for 72 h. Cell viability was measured by CCK-8 assay. Data represent the means±S.D. (n=3). (c) DLD-1 cells were treated with 5 μM LS-1-10 or 5 μM amsacrine for 48 h, and then subjected to flow cytometry to detect AnnexinV/propidium iodide staining. (d) Quantification of the apoptotic cells shown in (c). Data represent the means±S.D. (n=3). Student’s t-test, **P<0.01