Figure 3.

In vivo overexpression of miR-378 ameliorates the intestinal injury and cell apoptosis induced by intestinal I/R in TG mice. TG mice and WT mice were subjected to intestinal I/R injury. (a) RT-qPCR showed that the expression of miR-378 in TG mice was 3.39 times higher than that in the WT mice. (b) Histopathologic changes of intestinal mucosa in WT and TG mice subjected to I/R insult (hematoxylin and eosin staining, magnification × 100). (c) Injury scores for the intestinal mucosa morphology. (d) Intestinal cellular injury evaluated by serum DAO activity. (e and f) Representative TUNEL staining and the quantification of the apoptotic index. More apoptotic epithelial cells were observed at the villi in the WT injury group than in the miR-378 overexpression TG injury group. (g and h) Western blot analyses showing that overexpression of miR-378 in the TG mice led to lower cleaved caspase-3 expression after I/R insult. The data are expressed as the mean±S.D. (n=8). **P<0.01 compared with WT sham group, ^#P<0.05, ^##P<0.01 compared with WT injury group