Figure 4.

In vitro overexpression of miR-378 improves IEC-6 cell survival and inhibits caspase-3 activation after OGD/R challenge. (a) MiR-378 expression levels after transfection of miR-378 mimic, miR-378 inhibitor or their NCs. (b) Cultured IEC-6 cell injury was induced by depriving the culture media of oxygen and glucose (OGD), and cell viability was measured by MTT after transfection. (c) Representative Annexin V/PI dot plots of the flow cytometry analysis. The percentages of cells in the right upper and right lower quadrant (Annexin V-positive-staining cells represent apoptosis and necrosis) in each plot were analyzed. (d–f) MiR-378 mimic transfection resulted in significant downregulation of cleaved caspase-3 when compared with the OGD/R group, whereas pretreatment with miR-378 inhibitor further increased the cleaved caspase-3 expression. The data are expressed as the mean±S.D. (n=6). *P<0.05, **P<0.01 compared with the sham group; ^#P<0.05, ^##P<0.01 compared with OGD/R group