Figure 5.

The luciferase reporter assay of miR-378. MiR-378 mimic, miR-378 inhibitor and NC mimic were co-transfected with a modified control vector containing the caspase-3 3’-UTR. (a) A schematic representation of the interaction between miR-378 and the 3’-UTR of caspase-3. (b) The luciferase assay showed that miR-378 downregulated 46% of the expression of caspase-3 in the Reporter+mimic miR-378 group, whereas the miR-378 inhibitor reversed the effect of miR-378. The results indicated that miR-378 acts on the 3’-UTR. The data are expressed as the mean±S.D. (n=5 independent experiments). **P<0.01 compared with the Reporter+mimic NC group