Figure 1.

MBD2 is induced by VAN in HK-2 cells and mice nephrotoxic AKI. HK-2 cells were treated with 4 mm/l VAN for 0–24 h. Male C57BL/6 mice were (a-f) injected with 600 mg/kg VAN (n=8) for 0–7 days of examination. In a and b, cell lysates were analyzed by immunoblot with MBD2 and β-actin at the indicated time points (loading control). In c–f, kidneys were harvested for immunoblot analysis of MBD2, β-actin and immunohistochemistry staining of MBD2 at the indicated time points. β-Actin as an internal control. Scale bar, 100 μM. Data were expressed as means±S.D.; ^#P<0.05 versus day 0 or Saline group. Original magnification, × 200. Data are the representative of at least four separate experiments