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. 2017 Oct 26;8(10):e3154. doi: 10.1038/cddis.2017.525

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Copyright © 2017 The Author(s)

Cell Death and Disease is an open-access journal published by Nature Publishing Group. This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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The expression of miR-629 and FOXO3 in pancreatic carcinoma cells was negatively correlated. (a) Western blotting of FOXO3 in a human immortalized pancreatic ductal epithelial cell line HPDE6 and four pancreatic cancer cell lines (ASPC-1, Bxpc-3, Capan-2, and PANC-1) and the quantification of multiple blots; (b) Immunohistochemistry detection of FOXO3 in paraffin-embedded tissue sections and immunofluorescence staining of FOXO3 in Capan-2 (× 200). (c) Real-time RT-PCR detection of miR-629 and FOXO3 in pancreatic carcinoma tissues and their correlation. (d) Mean value of relative miR-629 expression by real-time RT-PCR in the 11 matched pancreatic carcinoma and normal pancreatic tissue (*P<0.01). (e) Real-time RT-PCR detection of miR-629 after transfection with miR-629 mimic or miR-629 inhibitor and the control sequence in Capan-2 cells. The results are normalized to U6B expression and are presented as relative miR-629 expression (*P<0.01)