Figure 5.

Overexpression of miR-629 promotes metastasis of pancreatic cancer cells in vitro and in vivo. (a) Transwell migration assays and Matrigel invasion assays showed inhibition of cellular invasion by overexpression of miR-629. Columns represent the means±S.D. *P<0.05. (b) The antitumor effect of miR-629 overexpression on pancreatic carcinoma xenografts. Two weeks postvector injection, tumor volume was imaged by Fluc bioluminescence imaging. (c) Macroscopic view of lung specimens of mice: there were many small nodules on the surface of the lung in miR-629 mimic group but it was smooth in the control group. Pulmonary metastatic tumor H&E staining (× 200): the metastasis nodi are indicated by arrows. (d) Tumor cells were injected subcutaneously into nude mice. The volume of each tumor was measured each week. Values represent means±SD. *P<0.05. Mice were killed after 4 weeks, and tumors were resected and weighed. Values represent means±S.D. *P<0.05