Figure 2.

Effect of conditional deletion (cKO) of Lkb1 in germ cells on spermatogenesis and epididymal sperm morphology. Testes were collected from control (con) and cKO mice for western blot and H&E staining at 5 weeks of age. Sperm were collected from cauda epididymides at 13 weeks of age for analysis. All experiments were replicated at least three times. (a) Significant decrease of Lkb1 proteins in Lkb1 cKO mice. (b) Sperm count from cauda epididymides. **P<0.01. (c) Representative tubules in 5-week-old control and cKO testes. Scale bar=50 μm. (d) Histology of epididymal tubules and sperm analysis. Scale bar=50 μm. (e) Spermatozoa morphology in control and cKO mice. Black arrows, abnormal multinucleated and multiflagellated sperm. Scale bar=50 μm. (f) Transmission electron microscopy of sperm in the epididymis. Black arrows and inset, multinucleated sperm. Scale bar=5 μm