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. 2017 Oct 12;8(10):e3121. doi: 10.1038/cddis.2017.527

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Copyright © 2017 The Author(s)

Cell Death and Disease is an open-access journal published by Nature Publishing Group. This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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Gradual germ cell loss in Lkb1 cKO mice. Testes were collected from control and cKO mice (n=3–5) at 5, 13, 20 and 40 weeks of age. (a) Testis gross appearance at 13 and 40 weeks of ages in both control and cKO mice. (b) Testis weight per body weight. (c) Histology of the seminiferous tubules. Scale bars=50 μm. Red asterisks, Sertoli only tubules. (d) Percentage of Sertoli only tubules in each section. (e) Upper panel, TUNEL staining of seminiferous tubules in 13W control and cKO mice. Lower panel, average number of apoptotic cells per tubule in 5- and 13-week-old testes by TUNEL staining. All data presented as mean±S.E.M. *P<0.05; **P<0.01