Figure 5.

Pro-apoptotic effect of K5 is mediated via downregulation of GRP78 in SGC-7901 cells under hypoxia. (a and b) SGC-7901 cells were transfected with siRNA specific to GRP78 or a scrambled control (siCTRL) for 24 h under normoxia and then incubated in hypoxic conditions for another 24 h. Apoptotic cells were quantified by flow cytometry. Colchicine treatment constituted positive control. **P<0.01 (a). Protein levels of GRP78 and caspase-7 were measured by western blotting (b). (c) SGC-7901 cells cultured with or without 640 nM K5 were exposed to 1% or 21% O₂ for 24 h prior to western blotting analysis of caspase-7 levels in total cell lysates.