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. 2017 Nov 13;3:17070. doi: 10.1038/cddiscovery.2017.70

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Copyright © 2017 The Author(s)

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Mitochondrial activity ceases following GSDMD pore formation. Fluorescent intensities of Sytox Blue, GFP, and tetramethylrhodamine methyl ester (TMRM) in GFP-expressing BMMs following exposure to RodTox±5 mM glycine. The start of GFP loss in the no-glycine condition is indicated with a vertical dotted line. Loss of GFP and TMRM fluorescence differ significantly±5 mM glycine (rANOVA, P<2E-16, P=0.0337, respectively). Data are pooled from six independent experiments and ‘n’ indicates total number of individual BMMs analyzed, with cells imaged every 45 s. Lines represent the population mean and shaded areas the 95% confidence interval. Influx of Sytox Blue into macrophages was set to time 0 : 00 to align traces. ‘−Gly’, no supplemental glycine; ‘+Gly’, 5 mM supplemental glycine.