TABLE 1.

ENZYME	Species	Gene Size (Kb)	Protein Size (kD)	PAM	DNA/RNA	Reference
SpCas9	Streptococcus Pyogenes	4.2	160	NGG	DNA	Jinek et al., 2012
SaCas9	Streptococcus aureus	3.3	105	NGRRT or NGRRN	DNA	Ran et al., 2016
CjCas9	Campylobacter jejuni	2.95	99	NNNNACAC or NNNNRY	DNA	Kim et al., 2017
FnCas9	Francisella Novicida	4.8	160	NGG	RNA	Hirano et al., 2016
Cpf1	Acidamino coccus sp.	3.9	130	TTTN or YTN	DNA	Zetsche et al., 2015
C2c2	Leptotrichia Shahii	4.3	140	Not Applicable	RNA	Abudayyeh et al., 2015
CasX	Plancto mycetes/Deltaproteo bacteria	2.94	98	TTCN	DNA	Burstein et al., 2017
CasY	Unknown	3.6	120	TA	DNA	Burstein et al., 2017

PAM is the protospacer adjacent motif.

N = A, C, G or T; R = C or G; Y = A or T