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. Author manuscript; available in PMC: 2018 Nov 1.
Published in final edited form as: Trends Genet. 2017 Sep 6;33(11):784–801. doi: 10.1016/j.tig.2017.07.006

Figure 6. A scheme for gene and enhancer trapping for the Gal4-UAS system.

Figure 6

A gene trap or an enhancer trap construct containing gal4 is injected into fertilized eggs with the transposase mRNA. Injected founder fish are raised and mated with UAS:GFP reporter fish. When gal4 is expressed under the control of an endogenous promoter/enhancer, GFP expression is induced in the F1 embryos via the Gal4-UAS system. Double transgenic F1 embryos express GFP in spatially and temporally restricted fashions may be picked up.