Table 1.
List of Antibodies Used in IHC, IF, and WB
| Antibody | Host | Class | Company (location) | Dilution | Application |
|---|---|---|---|---|---|
| Neutrophil (NIMP-R14) | Rat | Monoclonal | Abcam (Cambridge, MA) | 1:50 | IHC |
| CD4 | Rat | Monoclonal | Life Technologies Corp. (Carlsbad, CA) | 1:50 | IF |
| GFAP | Rabbit | Polyclonal | Dako Cytomation (Hamburg, Germany) | 1:50 | IF |
| CK19 | Goat | Polyclonal | Santa Cruz Biotechnology, Inc. (Dallas, TX) | 1:50 | IF |
| IFN-β | Rabbit | Polyclonal | Abcam | 1:200 | IF |
| Desmin | Mice | Monoclonal | Santa Cruz Biotechnology, Inc. | 1:500 | IF |
| Cleaved caspase-3 | Rabbit | Polyclonal | Cell Signaling (Beverly, MA) | 1:1000 | WB |
| JNK | Rabbit | Monoclonal | Cell Signaling | 1:1000 | WB |
| P-JNK | Rabbit | Monoclonal | Cell Signaling | 1:1000 | WB |
| IRF1 | Rabbit | Monoclonal | Cell Signaling | 1:1000 | WB |
| Histone H3 | Rabbit | Monoclonal | Cell Signaling | 1:2000 | WB |
| β-Actin | Mouse | Monoclonal | Sigma-Aldrich (St. Louis, MO) | 1:1000 | WB |
GFAP, glial fibrillary acidic protein; IF, immunofluorescence; IFN, interferon; IHC, immunohistochemistry; IRF1, interferon-regulatory factor-1; JNK, c-Jun-N-terminal kinase; P-JNK, phosphorylated JNK; WB, Western blot.