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. Author manuscript; available in PMC: 2018 May 15.
Published in final edited form as: Dev Biol. 2017 Mar 29;425(2):101–108. doi: 10.1016/j.ydbio.2017.03.017

Figure 2.

Figure 2

CNS angiogenesis and barriergenesis occur simultaneously. glut1b:mCherry; fli1a:EGFP double transgenic embryos were imaged by time-lapse confocal microscopy. Shown here are snapshots at 6-hour intervals beginning at the onset of CNS angiogenesis (30 hpf). Note that mCherry is immediately expressed as brain endothelial cells migrate into the brain parenchyma from the PHBCs (arrows in 36 hpf panels). See 30 hr time-lapse Videos 1 for more detail. MsV, mesencephalic vein; MCeV, mid-cerebral vein; PHBC, primordial hindbrain channel, E, eye. Scale bar in top left image is 50 μm for all images.