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. Author manuscript; available in PMC: 2018 Aug 29.
Published in final edited form as: Biochemistry. 2017 Aug 15;56(34):4489–4499. doi: 10.1021/acs.biochem.7b00451

Figure 5.

Figure 5

REP65-dependent production of 11-cis-retinol in the presence of LRAT(E14L). (A) HPLC analysis of retinoid composition extracted from NIH3T3 cells that stably express RPE65 and LRAT or its E14L mutant. Chromatography peaks were identified based on elution time and characteristic UV/vis absorbance spectra. Peak ‘a’ corresponds to 11-cis-retinol (UV/vis spectrum shown in panel B), whereas peak ‘b’ corresponds to 13-cis-retinol. (C) Time course of 11-cis-retinol production in NIH3T3-STRA6-LRAT (●) and NIH3T3-STAR6-LRAT(E14L) (○) cells. (D) Immunoblot analysis of expression of RPE65, LRAT, and its E14L mutant in NIH3T3 stable cell lines used for the 11-cis-retinol production assay.