Figure 2.

Src kinase mediates accelerated prostate tumor growth by dietary fat. A, PC-3 cells were transduced with shRNA-Control or shRNA-Src and subcutaneously injected into both flanks of SCID mice (n = 6/group). The mice were placed on a 10% or 45% fat diet for 8 weeks. Representative images of mice and of excised xenografts are shown (scale bar = 8 mm). B, the size and weight of xenografts are represented as mean ± S.E. (n = 6/group). Statistics were performed using two-way analysis of variance by the SAS system. C–F, RFP fluorescence and IHC staining of total Src kinase, pSrc(Tyr-416), and pErk1/2 of PC-3 xenografts from A (scale bars = 200 μm). G and H, the expression levels of pSrc and pErk in E and F were analyzed by image analysis software. #, p < 0.05; ##, p < 0.01; ###, p < 0.005; N.S., not significant.