Figure 6.

Hyperphosphorylation requires Cdr1 kinase activity. A, α-FLAG Western blot of whole-cell extracts from the indicated strains after KCl treatment. K41A is kinase-dead mutation of Cdr1 (20, 21). B, Cdr1(K41A)-GFPγ does not exit nodes during osmotic stress. Shown are middle focal plane inverted images of a time course with or without 1 m KCl. Insets show enlarged images of Cdr1 present at nodes; yellow boxes indicate the enlarged area. Scale bar = 5 μm. C, quantification of cells with Cdr1(K41A)-GFPγ localization at nodes (n > 100 for each time point, error bars represent standard deviation).