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ES7c cleavage is not part of the UPR. Overnight cultures from the indicated strains were diluted with YPDA to an A600 of ∼0.3, grown for 2 h, and treated for 2 h with 20 mm DTT or 1 μg/ml Tm or left untreated (−). RNA was extracted, resolved on a gel in duplicate, and hybridized with the 25S rRNA probe y540 (top panel) and a probe that recognizes spliced (HAC1i) and unspliced (HAC1u) forms of HAC1 (bottom panel).
