Figure 6.

ES7c cleavage coincides with activation of the thioredoxin system during low-dose oxidative stress. A, wild-type overnight culture was diluted with YPDA to an A₆₀₀ of ∼0.2, grown for 4 h and treated with 0.2 mm H₂O₂ for 30 min or 2 mm H₂O₂ for 2 h. RNA was extracted from the cells, and the levels of TRX2, TRR1, CTT1, and CTA1 mRNAs were analyzed by qRT-PCR. Transcript levels relative to the ACT1 mRNA are shown as mean values normalized to no-treatment conditions (dashed horizontal line, y = 1) in triplicate samples; the error bars show S.D. B, overnight cultures of wild-type and yap1Δ strains were analyzed as in A after treatment with 0.2 mm H₂O₂ for the indicated times. C, Northern hybridizations of the rRNA from cultures shown in B. D, ES7c-cleaved fragments from samples treated as in B and C were quantified as described in Fig. 3B. The data show mean values of three independent experiments; the error bars represent S.D.