Table 1.

Concentration of compounds used in different treatments

PEDF was purchased from Bio Products (Middletown, MD). DHA and atglistatin (a PEDF-R inhibitor) were purchased from Cayman Chemical (Ann Arbor, MI). The 44-mer PEDF peptide was prepared by GenWay Biotech, Inc. (San Diego). NPD1 was a kind gift from Dr. Nicos A. Petasis, Loker Hydrocarbon Research Institute, University of Southern California, Los Angeles.

Experiment (Figs.)	Compounds	Kind of treatment
		Topical (10 μl/eye)	i.p.	Ex vivo organ culture
		Ng	μmol/kg
1–6	PEDF	0.5		50 ng/ml
1–6	DHA	100		50 nm
4	44-mer PEDF	0.05		5 ng/ml
4	NPD1	10		50 nm
5, 6	Atglistatin	23.7	200