Table 2.
Primer sequences for gene expression analysis by q-PCR in the cornea
Three housekeeping genes, which were used to normalize the gene expression level, are shown in boldface in the table.
| Gene | Name | Primer sequence |
|
|---|---|---|---|
| Forward | Reverse | ||
| bdnf | Brain-derived neurotrophic factor | TTGTTTTGTGCCGTTTACCA | GGTAAGAGAGCCAGCCACTG |
| cd40 | CD40 molecule | TTGTTGACAGCGGTCCATCTA | GCCATCGTGGAGGTACTGTTT |
| cntf | Ciliary neurotrophic factor | TCTGTAGCCGCTCTATCTGG | GGTACACCATCCACTGAGTCAA |
| fgf2 | Basic fibroblast growth factor | GCGACCCACACGTCAAACTA | TCCCTTGATAGACACAACTCCTC |
| gdnf | Glial cell-derived neurotrophic factor | TCTTTCGATATTGCAGCGGTT | GTCACTTGTTAGCCTTCTACTCC |
| ngf | Nerve growth factor | TTTGGAAACTCCTAGTGAACA | GTATAGAAAGCTGCGTCCTT |
| npy | Neuropeptide Y | ATGCTAGGTAACAAGCGAATGG | TGTCGCAGAGCGGAGTAGTAT |
| nrg1 | Neuregulin 1 | GAGGTGAGAACACCCAAGTCA | TGGTCCCAGTCGTGGATGTAG |
| sema7a | Semaphorin 7A | GGTGATGTCACTTGGTGAGATG | TCTTCCCGTTGTATTCCTGCT |
| tacr1 | Tachykinin receptor 1 | CTCCACCAACACTTCTGAGTC | TCACCACTGTATTGAATGCAGC |
| actb | Actin β | GTGACGTTGACATCCGTAAAGA | GCCGGACTCATCGTACTCC |
| gapdh | Glyceraldehyde-3-phosphate dehydrogenase | AGGTCGGTGTGAACGGATTTG | TGTAGACCATGTAGTTGAGGTCA |
| tbp | TATA box-binding protein | AGAACAATCCAGACTAGCAGCA | GGGAACTTCACATCACAGCTC |