Table 4.
Primer sequences for gene expression analysis by qPCR in the trigeminal ganglia
Three housekeeping genes, which were used to normalize the gene expression level, are shown in boldface in the table.
| Gene | Name | Primer sequence |
|
|---|---|---|---|
| Forward | Reverse | ||
| calca | Calcitonin-related polypeptide α | CAGTGCCTTTGAGGTCAATCT | CCAGCAGGCGAACTTCTTCTT |
| calclr | Calcitonin gene-related peptide receptors | ATCTCAGCAGAGTCGGAAGAA | CAGGTCCTATTGCAGTAAAGGC |
| ngfr | Nerve growth factor receptor | CTAGGGGTGTCCTTTGGAGGT | CAGGGTTCACACACGGTCT |
| npy | Neuropeptide Y | ATGCTAGGTAACAAGCGAATGG | TGTCGCAGAGCGGAGTAGTAT |
| npy1r | Neuropeptide Y receptor 1 | TGATCTCCACCTGCGTCAAC | ATGGCTATGGTCTCGTAGTCAT |
| npy2r | Neuropeptide Y receptor 2 | TCCGGGAATACTCCCTGATTG | GCAAAACGTACAGGATGAGCAG |
| npy5r | Neuropeptide Y receptor 5 | TTTGTCACGGAGAACAATACTGC | TGCGCTTTTTCATAACAGCCAT |
| sprr1a | Small proline-rich protein 1A | TTGTGCCCCCAAAACCAAG | GGCTCTGGTGCCTTAGGTTG |
| tac1 | Tachykinin precursor 1 | ATTCCTTTGTTGGACTAATGGGC | ACGTCTTCTTTCGTAGTTCTGC |
| tacr1 | Tachykinin receptor 1 | CTCCACCAACACTTCTGAGTC | TCACCACTGTATTGAATGCAGC |
| trka | Neurotrophic receptor tyrosine kinase 1 | CAGTCTGATGACTTCGTTGATGC | CTCTTCACGATGGTTAGGCTTC |
| trkb | Neurotrophic receptor tyrosine kinase 2 | GTTGACCCGGAGAACATCACG | ACTTTAAGCCGGAATCCACAAT |
| vip | Vasoactive intestinal peptide | AGTGTGCTGTTCTCTCAGTCG | GCCATTTTCTGCTAAGGGATTCT |
| vipr2 | Vasoactive intestinal peptide receptor 2 | AGGCCATTTATACCTTGGGCT | GCAGTAGACCTGAGCTGGAGTA |
| tubb3 | Tubulin β-III | CGCACGACATCTAGGACTGA | TGAGGCCTCCTCTCACAAGT |
| gapdh | Glyceraldehyde-3-phosphate dehydrogenase | AGGTCGGTGTGAACGGATTTG | TGTAGACCATGTAGTTGAGGTCA |
| tbp | TATA box-binding protein | AGAACAATCCAGACTAGCAGCA | GGGAACTTCACATCACAGCTC |