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. 2017 Sep 22;292(45):18500–18517. doi: 10.1074/jbc.M117.811356

Figure 8.

Figure 8.

Guanine nucleotide dependence of Rab11a-mediated membrane tethering in the presence of Myo5A-GTD and Myo5B-GTD. A, schematic representation of liposome turbidity assays for testing Rab11a- and Myo5-GTD–dependent liposome tethering in the presence of GTP (shown in B–G). B and C, Rab11a/Myo5-dependent membrane tethering is strongly and specifically promoted by the addition of GTP. Liposome turbidity assays with Rab11a-His12 (1 μm) and Myo5A-GTD (1 μm) (B) or Myo5B-GTD (2 μm) (C) were performed as described in the legend for Fig. 7, D and E, but in the presence of GTP (1 mm). D and E, liposome turbidity assays were employed with Rab11a-His12 and Myo5A-GTD (D) or Myo5B-GTD (E), as described in B and C, in the presence of various concentrations of GTP (1 μm–1 mm). F and G, liposome turbidity assays were employed with Rab11a-His12 and Myo5A-GTD (F) or Myo5B-GTD (G), as described in B and C, in the presence of GTP, GTPγS, and GDP (each at 1 mm).