Figure 7.

The LXRE region of the glut4 promoter is required for glucose-dependent differentiation. Shown is a schematic of the luciferase reporter gene under the regulator control of two different glut4 promotors, differing only by their inclusion of the LXRE region. 3T3-L1 adipocytes were induced for differentiation and pulsed with Hi glucose, Lo glucose, or Pyr and cultured in Lo glucose medium during the P and M phases of their cell culture. Day 6 adipocytes were collected and lysed accordingly, and the supernatants were analyzed for luciferase expression. 95% confidence intervals were used to determine statistical differences (p < 0.05 for n = 3 independent experiments. *, differences compared with Hi glucose cells; #, differences compared with Lo glucose cells with the same glut4 promoter region.