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. 2017 Sep 22;292(45):18592–18607. doi: 10.1074/jbc.M117.799676

Figure 1.

Figure 1.

Brg1 is phosphorylated in vitro by CK2. A, schematic representation of the location of the putative sites for CK2 phosphorylation identified by NetphosK (9, 20). B, representative autoradiograms of in vitro CK2-treated WT-, SA-, and SE-Brg1 (top). A representative Western blot detecting Brg1 is shown for comparison (bottom). EV, empty vector. C, Brg1 labeling was quantified by normalizing autoradiography signals to the Western blotting for Brg1. Data represent the average of three independent experiments ± S.D.; *, p < 0.01. Phosphorylation in the WT-Brg1-expressing cells was set at 1. A.U., arbitrary units. D, diagram of the GST-Brg1 fragments (#1–6) used to characterize the phosphorylation by CK2 relative to the predicted CK2 sites and the known domains of Brg1. E, representative autoradiogram of in vitro CK2-labeled GST-Brg1 fragments (top). Representative Coomassie Brilliant Blue (CBB) staining is shown for comparison (bottom).