Table 2.

Primers used in this study

oePCR, overlap extension PCR.

Name	Sequence	Restriction site	Purpose
PfA506-4424-F1-MR	ATAGCCGGATCCTAGACAGGTAGCGCCAAATCACG	BamHI	Forward primer for ptaA left flanking region
PfA506-4424-R1-MR	AGGAGTAGTCACCATGGTGCGTGTCACGCAGGTGGTCTGATCAGGCGGCGCTATAGCTG		Reverse primer for ptaA left flanking region
PfA506-4424-F2-MR	GACACGCACCATGGTGACTACTCCTTG		Forward primer for ptaA right flanking region
PfA506-4424-R2-MR	GGCCGCGAATTCTTTGTACAGATTCTTGATTTCCATCTTG	EcoRI	Reverse primer for ptaA right flanking region
PfA506-4424-DF-MR	TTTGAGTGCTGCGCCTATTG		ptaA genomic deletion control primer
PfA506-4424-DR-MR	CGCGGCAATGCTGGTGAAGATATAG		ptaA genomic deletion control primer
PfA506-4424-F-MR	TCATCGCATATGGTGCGTGTCAGTCGTCGATCC	NdeI	Forward primer for cloning PtaA-coding region into pEXH5
PfA506-4424-strep-R-MR	GGCCGCAAGCTTTTACTTTTCGAACTGCGGGTGGCTCCAGACCACCTGCGTCGCAAAGGCCTCGC	HindIII	Reverse primer for cloning PtaA-coding region into pEXH5
PfA506-4424_K224A-F-MR	CTGGTGCTGCGCACCTTCTCCGCCATCTAC		Primer for K224A exchange by oePCR
PfA506-4424_K224A-R-MR	CCGGCCATGCCGTAGATGGCGGAGAAGGTG		Primer for K224A exchange by oePCR
PfA506-mat4424-F-MR	GATATACATATGAGCCCAGCGCCGACAAAATCTGACC	NdeI	Forward primer for cloning mature PtaA-coding region into pEXH5
pEXH5-RBS-F-MR	GGCGCGGGATCCGTTTAACTTTAAGAAGGAGATATAC	BamHI	Forward primer for subcloning from pEXH5 into pME6010
pEXH5-strep-term-HindIII-R-MR	CCCCTTAAGCTTAAAAAAAACCCCGCCCTGTCAGGGGCGGGGTTTTTTTTTTTACTTTTCGAACTGCGGGTGGCTCC	HindIII	Reverse primer for subcloning from pEXH5 into pME6010