Figure 3.

Deoxynucleotide incorporation by Polι(1–452) opposite undamaged A and 3-dMeA. Polι (0.2 nm) was incubated with DNA (10 nm) and with 50 μm of one of the 4 dNTPS (A, T, G, or C) or with 50 μm of each of 4 dNTPS (N) for 10 min at 37 °C. The DNA substrate is shown above the gel; the asterisk indicates the site of an A or 3-dMeA.