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. 2017 Oct 26;73(Pt 11):921–939. doi: 10.1107/S2059798317015236

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© Radka et al. 2017

This is an open-access article distributed under the terms of the Creative Commons Attribution (CC-BY) Licence, which permits unrestricted use, distribution, and reproduction in any medium, provided the original authors and source are cited.

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Purification and model fit of YiuA. (a) SDS–PAGE gel showing the enrichment of YiuA over the various steps of purification. Molecular-weight standards are shown on the left (labeled in kDa). Lane 1, uninduced BL21 whole cells. Lane 2, BL21 whole cells induced with IPTG. Lane 3, lysate supernatant fraction following French press cell disruption. Lane 4, Ni-affinity chromatography eluate. Lane 5, anion-exchange chromatography eluate. Lane 6, gel-filtration peak fraction. (b) HiLoad 26/600 Superdex 200 pg gel-filtration chromatogram for YiuA purification. Fractions containing the peak from this chromatogram were concentrated, are represented in lane 6 in (a) and were used for crystallography. (c) Model overlay of 2F _o − F _c electron difference density (bright blue mesh) at site 1.