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. 2017 Oct 26;73(Pt 11):921–939. doi: 10.1107/S2059798317015236

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© Radka et al. 2017

This is an open-access article distributed under the terms of the Creative Commons Attribution (CC-BY) Licence, which permits unrestricted use, distribution, and reproduction in any medium, provided the original authors and source are cited.

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Loci acclimation in vitro. E. coli expressing the Yiu transporter require a threefold greater time to acclimate under nutrient-limiting conditions than E. coli expressing the Yfe transporter. (a, b, c) Periplasmic fractions of E. coli constructs growing in various in vitro media conditions. Black arrows denote the positions of electrophoretic migration for YfeA (b) and YiuA (c). Lanes 1–4 represent time points 3, 5, 7 and 10 h from M9 minimal medium base conditions. Lanes 5–8 represent time points 3, 5, 7 and 10 h from M9 minimal medium with 5 µM Fe₂(SO₄)₃ supplementation. Lanes 9–12 represent time points 3, 5, 7 and 10 h from M9 minimal medium with 1 mM EDDA supplementation. Molecular-weight markers are labeled in kDa. (d, e, f) Growth curves for the pET-22b construct (d), pYFE3 construct (e) and pYIU3 construct (f). Error bars represent the standard deviation in OD₆₀₀ from experiments performed in triplicate.