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| Method | Hanging-drop vapour diffusion |
| Plate type | Hampton Research 48-well plates |
| Temperature (K) | 293 |
| Protein concentration (mg ml−1) | 9 |
| Peptide (Lys-Pro-Tyr) concentration (mM) | 4 |
| Buffer composition of protein solution | 80 mM Tris–HCl pH 8.5 |
| Composition of reservoir solution | 80 mM MES pH 6.5, 12%(w/v) PEG 20 000, 20%(v/v) glycerol |
| Volume of drop | 1 µl protein + 1 µl reservoir |
| Volume of reservoir (µl) | 200 |
