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. 2017 Oct 23;73(Pt 11):607–611. doi: 10.1107/S2053230X17015102

Table 1. Macromolecule-production information.

Source organism Bacillus sp. HJB17
DNA source GenBank accession No. HM003049.1
Forward primer 5′-GCCCGGAATTCGGCAGTCCAACCTTTATCGGTC-3′
Reverse primer 5′-GCCGTGCTCGAGCTCCGGCTTCGCCTGCAGCAC-3′
Cloning vector pET-22b(+)
Expression vector pET-22b(+)
Expression host E. coli BL21 (DE3)
Complete amino-acid sequence of the construct produced MDIGINSDPNSAVQPLSVNASHFHKVQLAGQDYQLFTTTTALQIRQGNRELAQQAGQFSRLVLQPLDTDALLLAAMDINSNTLFLWRFSTKQTPSLQLLQRRLISSRVVDDLCFYHSTENQQLSLFLLGGRGGADQLLLQQQQQWLAQPVVIRELNIPYDSTACVVDQTAGALYIAEADRAIWRYQAEPEADEGRSLVQVNKPFGQLQGEVKALQTLSDGSLLALEEAPARLLHINSDGQLRSAIAVPALAEASGLAVSMQGNTATAYISTEDAGAVQQLAVVLQAKPELEHHHHHH