Table 1. Macromolecule-production information.
Source organism | Bacillus sp. HJB17 |
DNA source | GenBank accession No. HM003049.1 |
Forward primer | 5′-GCCCGGAATTCGGCAGTCCAACCTTTATCGGTC-3′ |
Reverse primer | 5′-GCCGTGCTCGAGCTCCGGCTTCGCCTGCAGCAC-3′ |
Cloning vector | pET-22b(+) |
Expression vector | pET-22b(+) |
Expression host | E. coli BL21 (DE3) |
Complete amino-acid sequence of the construct produced | MDIGINSDPNSAVQPLSVNASHFHKVQLAGQDYQLFTTTTALQIRQGNRELAQQAGQFSRLVLQPLDTDALLLAAMDINSNTLFLWRFSTKQTPSLQLLQRRLISSRVVDDLCFYHSTENQQLSLFLLGGRGGADQLLLQQQQQWLAQPVVIRELNIPYDSTACVVDQTAGALYIAEADRAIWRYQAEPEADEGRSLVQVNKPFGQLQGEVKALQTLSDGSLLALEEAPARLLHINSDGQLRSAIAVPALAEASGLAVSMQGNTATAYISTEDAGAVQQLAVVLQAKPELEHHHHHH |