Table 2. Crystallization.

Method	Vapour diffusion
Plate type	48-well Double Sample Crystallization Plate (screening), 16-well Crystallization Plate (final)
Temperature (K)	289
Protein concentration (mg ml−1)	1–5
Buffer composition of protein solution	20 mM Tris pH 8.0, 100 mM NaCl
Composition of reservoir solution	0.2 M sodium chloride, 0.1 M Tris pH 8.5, 25%(w/v) PEG 3350 (screening); 0.2 M sodium chloride, 0.1 M Tris pH 9.0, 30%(w/v) PEG 3350 (final)
Volume and ratio of drop (µl)	2, 1:1
Volume of reservoir (µl)	80 (screening), 400 (final)