Fig. 3.
Inhibitory effect of MMPP on LPS-induced expression of Aβ1-42 in both GFAP and Iba-1-positive mice brain. Staining was performed in 20-μm-thick sections of mice brain. Confocal microscope observation was performed as described in the Methods section. Immunostaining of GFAP (green) and Aβ1−42 (red) protein in the hippocampus was performed with specific primary antibodies, and fluorescence was developed using Alexa 488-conjugated anti-goat and Alexa 568-conjugated anti-rabbit secondary antibodies (a). Iba-1 (red) and Aβ1−42 (green) protein in the hippocampus was performed with specific primary antibodies, and fluorescence was developed using Alexa 488-conjugated anti-mouse and Alexa 568-conjugated anti-rabbit secondary antibodies (b). Similar patterns were observed in five mice brain