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. Author manuscript; available in PMC: 2018 Jul 4.
Published in final edited form as: Free Radic Res. 2017 Jul 4;51(6):582–590. doi: 10.1080/10715762.2017.1339303

Figure 1. GPX enzyme activity and MnSOD levels are expressed in MCF-7 transfectants.

Figure 1

A. Lysates from MCF-7 transfected with the empty vector (C), MCF-7 GPX1, MCF-7 MnSODVal and MCF-7 MnSODAla cell lines were used to determine GPX enzyme activity using a spectrophotometric assay that determines the GPX dependent consumption of NADPH. B. Lysates from MCF-7, GPX1, MnSODVal, and MnSODAla cells were analyzed for MnSOD protein levels by western blot using anti-MnSOD antibodies. β-Actin was used as an endogenous protein loading control. Protein levels were quantified using fluorescence detection and normalized to β-Actin. Error bars indicate the standard deviation (n=3) (* = p<0.05).