Fig. 3.
Neuronal miR insertion exerts specific effects on peripheral replication and neuropathogenesis. Mice were inoculated IP at 1000 IU with WT WNV, miR-14, and miR-124a. Mice were either euthanized for serum viremia (A, n = 3 per virus per time point) or held for survival analysis for 21 days (B, n = 15). IC-inoculated mice were assayed for neuronal infection by histologic characterization (C). Top row: H&E; bottom row: immunohistochemistry. Human neuronal SH-SY5Y cells were inoculated with these viruses at an MOI of 0.1 and assayed for viral titer daily through 6 days post infection (D).