Fig. 5.

PRNT₉₀ titers in mice after vaccination and after challenge. (A) Mouse sera were tested by PRNT₉₀ assay at 21 days post vaccination (circles) then challenged, and serum was tested subsequently by PRNT₉₀ at 21 days post challenge (triangles). Each stacked pair represents an individual mouse and the x-axis distribution for each graph represents individual mice from 1 (far left) to 15 (far right). Mice whose titers fell below the limit of detection (20 PRNT₉₀, ~4.5 log₂ PRNT₉₀) are reported here at the limit of detection, indicated by the dotted line. (B) Fold-change titers from Fig. 4A from 21 days post-vaccination to 21 days post-challenge were compared. Green bars represent groups of animals whose titers increased (>1-fold-change), grey bars represent groups of animals with no change in titer (=1-fold-change), and blue bars represent groups of animals with a decrease in titer (<1-fold-change) after challenge. (For interpretation of the references to color in this figure legend, the reader is referred to the web version of this article.)