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. Author manuscript; available in PMC: 2017 Nov 13.
Published in final edited form as: Cell Rep. 2017 Feb 14;18(7):1751–1760. doi: 10.1016/j.celrep.2017.01.056

Figure 1. Experimental Design.

Figure 1

(A) Fourteen 18-week-old (wko) male AG129 mice were inoculated i.p. and mated to female CD-1 mice daily to collect seminal fluids until the male mice were euthanized due to disease progression.

(B) Eleven 13-week-old male AG129 mice were inoculated i.p., mated to female AG129 mice for sexual transmission studies, and then mated to female CD-1 mice daily to collect seminal fluids until the male mice were euthanized.

(C) Eight 18-week-old AG129 male mice were vasectomized before intraperitoneal inoculation and then mated to female CD-1 and AG129 mice to collect seminal fluids and assess sexual transmission until the male mice were euthanized.

(D) Eighteen 8-week-old male AG129 mice were inoculated i.p. and euthanized in groups of three at prescribed days 1, 3, 5, 7, 9, and 11 post-inoculation.

(E) Eight 13-week-old female AG129 mice were inoculated i.p. and mated to male AG129 mice for sexual transmission studies.