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. 2017 Sep 18;292(45):18713–18728. doi: 10.1074/jbc.M117.809970

Table 2.

Plasmids used

Plasmid Genotype/description Source/reference
pFA6a-GFP(S65T)-kanMX Kanamycin resistance cassette for genomic N terminus tagging of GFP Ref. 69
pCRCU[TGL4-RFP] URA3 cassette with TGL4 cloned at NotI-ClaI Gift of S. Kohlwein
pBP73-C pRS416 with the CPY1 promotor cloned at SacI-XbaI and GFP at Xba-BamHI Ref. 72
pBP73-C[GFP-OPI1] pBP73-C with OPI1 cloned at BamHI-HindIII This study
pBP73-C[GFP-OPI1ffat] pBP73-C with OPI1ffat cloned at BamHI-HindIII This study