Table 2.
Plasmids used
Plasmid | Genotype/description | Source/reference |
---|---|---|
pFA6a-GFP(S65T)-kanMX | Kanamycin resistance cassette for genomic N terminus tagging of GFP | Ref. 69 |
pCRCU[TGL4-RFP] | URA3 cassette with TGL4 cloned at NotI-ClaI | Gift of S. Kohlwein |
pBP73-C | pRS416 with the CPY1 promotor cloned at SacI-XbaI and GFP at Xba-BamHI | Ref. 72 |
pBP73-C[GFP-OPI1] | pBP73-C with OPI1 cloned at BamHI-HindIII | This study |
pBP73-C[GFP-OPI1ffat] | pBP73-C with OPI1ffat cloned at BamHI-HindIII | This study |