Table 2.
Sequences and properties of the primers used in the study
Gene | Primer 5′ - 3′ | Amplicon length (bp) |
Transcript variants | Encoded Isoforms |
---|---|---|---|---|
ESR2 | F:AATTGACCACCCCGGCAAG | 64 | a, b, d, f, k and l | ERβ1, ERβ2 and ERβ4 |
Primers 1 | R: TTTCCCCTCATCCCTGTCCA | |||
ESR2 | F:GGCTAACCTCCTGATGCTCC | 57 | a and g | ERβ1 and ERβ5 |
Primers 2 | R: TCCATGCCCTTGTTACTCGC | |||
ESR2 a | F:TCTCCTCCCAGCAGCAATCC | 154 | b, l and k | ERβ 2 |
Primers 3 | R:GGTCACTGCTCCATCGTTGC | |||
PUM1 | F:CACAGACACCACCTCCTTCC | 73 | ||
R:CCATTCGTGAGTCCTCCCAG | ||||
EIF2B1 | F:ACCTGTCTTCATCCTCCCCT | 71 | ||
R:GCTGCTTTTCGCCTGCATC | ||||
POP4 | F:TTACCTGCTTTCCCGCTGAG | 114 | ||
R:GGCTAGGAAGCTACAGCACC |
bp: base pairs aThis primer sequence was obtained from ref. [22] F: forward; R: reverse