Table 1.

pCS20 Sol1^TqM qPCR sensitivity and specificity test on Ehrlichia ruminantium strains and other tick-borne pathogens

Name	Origin	Geographical origin	Nested pCS20 PCR	Sol1TqM QPCR	Reference
E. ruminantium strains
Blonde	CC p8	Guadeloupe	+	+	[31]
Gardel	CC p48		+	+	[29]
Sara 455	CC p10	Burkina Faso	+	+	[8]
Bekuy 255	CC p9		+	+	[31]
Bankouma 421	CC p15		+	+	[8]
Banankeledaga	CC p1		+	w+	[8]
Lamba 479	CC p16		+	+	[31]
Cameroun	CC p9	Cameroon	+	+	[31]
Pokoase 412	CC p10	Ghana	+	+	[31]
Senegal	CC p60	Senegal	+	+	[31]
Umbaneim	B	Sudan	+	+	[31]
Mara	CC p1	South Africa	+	+	[31]
Welgevonden	CC p12		+	+	[29]
Lutale	CC p6	Zambia	+	+	[31]
Sankat 430	CC p16	Ghana	+	+	[32]
Umpala	CC p6	Mozambique	+	+	[31]
MAT2-17MH1	T		+	w+	[33]
MAT2-26MH1			+	+	[33]
MAT2-32MH2			+	w+	[33]
CHOB4MH2			+	w +	[33]
CHOB25MH1			+	w+	[33]
CHA2-32MH1			+	w+	[33]
KNP51MH1	T	South Africa	+	w+	[33]
KNPC15MH1			w+	w+	[33]
KNPC2MH1			+	+	[33]
KNPC2MH2			+	+	[33]
Other tick-borne pathogens
A. marginale		Argentina	–	–
A. phagocytophilum	CC	USA	–	–
A. platys (E. platys)	CC		–	–
B. bovis		Argentina	–	–
B. bigemina			–	–
E. canis	CC	USA	–	–	[34]
E. muris	CC		–	–	[35]
PME 160055	T		+	–
PME 160178			–	–
PME 160277			+	–
PME 160359			–	–
PME 160366			+	–
PME 160491			+	–
R. felis	CC		–	–	[36]
R. parkeri	CC		–	–	[37]
9 uninfected A. variegatum	T	Guadeloupe	–	–

E. ruminantium was detected from A. variegatum and A. hebraeum ticks while PME was detected from A. americanum ticks

Abbreviations: T ticks, B blood, CC p cell culture passage, + positive, − negative, w + weak positive