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Relative sensitivity and specificity of automated extraction + pCS20 Sol1TqM qPCR compared with manual extraction + pCS20 nested PCR. Results obtained on 17 E. ruminantium serial dilutions and 30 experimentally infected ticks. The kappa test for this analysis was 49%
| Manual extraction + pCS20 nested PCR | Total | Se (%) | Sp (%) | Ac (%) | |||
|---|---|---|---|---|---|---|---|
| + | – | ||||||
| Automated extraction +Sol1TqM qPCR | + | 16 | 7 | 23 | 76.2 | 73.1 | 74.5 |
| – | 5 | 19 | 24 | ||||
| Total | 21 | 26 | 47 | ||||
Se relative sensitivity, Sp relative specificity, Ac accuracy
