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. Author manuscript; available in PMC: 2018 Oct 1.
Published in final edited form as: Circ Cardiovasc Genet. 2017 Oct;10(5):e001678. doi: 10.1161/CIRCGENETICS.116.001678

Figure 2.

Figure 2

Smoking-Related miRNAs Alter Cytokine Expression in Vitro. Multiplex cytokine bead array analysis of A549 cell culture supernatants after transfection with non-targeting (NT), miR-1180, or miR-1285-3p mimetics. Cells were stimulated 24 hours post-transfection with vehicle or 10 ng/mL rhTNF-α for 6 hours. Squares, triangles, and circles represent data collected from three independent experiments, normalized to the cytokine concentrations in the negative control group (NT vehicle). The * indicates p<0.01 (significant p-values shown) when compared to NT control within a given stimulation group as determined by a two-way ANOVA followed by Dunnett’s test, using raw rather than normalized values.