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. 2017 Nov 13;5:83. doi: 10.1186/s40478-017-0486-9

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© The Author(s). 2017

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 3 — Axonal arborization defects in G607S hindlimb and foot muscles. Motor nerves were visualized by GFP immunoreactivity to compare the innervation pattern between E17.5 wild-type (a) and homozygous knock-in mutant (b, c) foot muscles. The terminal number of motor nerves is individually plotted. Black bars represent the median. The number of motor nerve terminals was significantly reduced in both G607S and C760R mutant muscles (d). Kruskal-Wallis test followed by the Steel-Dwass test, *p < 0.05. n = 5. Scale bar: 100 μm (a–c)