Fig. 5.

Expression analyses of DINE in G607S mutant spinal cords. Immunohistochemical analysis with anti-DINE antibody in horizontal sections of E12.5 mouse spinal cords from wild-type (a–c), homozygous G607S mutant (d–f) and DINE-deficient embryos (g–i). j Western blotting analysis using spinal cord protein from wild-type, homozygous G607S mutant, and DINE-deficient embryos. k Quantitative evaluation of the western blotting. Two-tailed Student’s t test, **p < 0.01. l Quantitative expression analysis using spinal cord mRNA from wild-type and homozygous G607S mutant embryos. Two-tailed Student’s t test, **p < 0.01. m Schematic image of qualitative RT-PCR analysis for the mutant transcript. n Wild-type and mutant transcripts were evaluated by RT-PCR. The arrow and arrowhead indicate the size of the pre-mRNA and mRNA products, respectively. o Electropherograms showing the sequencing results of RT-PCR products from wild-type (left) and homozygous G607S mutant (right). Scale bar: 200 μm (a–i). SC, spinal cord