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. 2017 Nov 13;10:593. doi: 10.1186/s13104-017-2913-1

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© The Author(s) 2017

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 2 — Dilution series: DNA concentration in the PCR mix is serialy diluted by a factor 5, six times. The DNA concentration is measured using the robust regression method and the $Δ C_{T}$ method. Each concentration is prepared in 16 replicas. The same procedure is reproduced for both nuclear and mitochondrial DNA. Regression lines are fitted using the least squared method. The data has been shifted slightly left and right of the true value for illustration purposes