Fig. 4.

The number of intracellular bacteria and the cell viability over time after infection with various bacteria. THP-1 macrophages were infected for 2, 4, 6, and 8 h with NTHi, Mrc, Spn or Psa, then washed, treated with 300 μg gentamicin and lysed to determine the number of intracellular bacteria (a). The viability of THP-1 macrophages was determined over the course of infection (b). Data are represented as the mean ± SEM (n = 3). *p < 0.05, **p < 0.01