Fig. 2.

a Different time points of the in vitro growth of the CAISMOV24 cell line. CAISMOV24 cells were launched at 10⁴ cells/cm² in HAM F10 medium supplemented with 2 mM L-glutamine and 10% fetal bovine serum. b Representative growth curve for the CAISMOV24 cell line, assessed from the 63rd to the 100th in vitro passages. c Proliferation profile of CAISMOV24 cells assessed by flow cytometry on day 5, following cell labeling with violet proliferation dye 450 (VPD450); the shaded areas represent each of the new cell generations, which retained approximately half of the VPD450 fluorescence intensity of its parent cell. Mean proliferation index of CAISMOV24 cells resulted in 3.94 ± 0.94 times