Fig 3. Effect of pH on the activity of different recombinant CPD forms.

(A) Effect of pH on rhCPD and (B) single point mutants using 200 μM dansyl-Phe-Ala-Arg in a Tris-acetate buffer at the indicated pH for 60 min at 37°C. The activity represented is the average of three independent measures with less than a 10% of variation, expressed as a percentage of the maximal activity, observed at optimal pH.