Table 1. Kinetic constants for hydrolysis of dansyl-Phe-Ala-Arg by rhCPD and rhCPD single point mutants.

Substrate	rhCPD	Active domain I rhCPD (E762Q)	Active domain II rhCPD (E350Q)	Double mutant rhCPD (E762Q /E350Q)
Dansyl-Phe-Ala-Arg
km (μM)	153 ± 16	319 ± 37	844 ± 139	ND
kcat (s-1)	12.5 ± 0.5	8.5 ± 0.5	7.0 ± 0.9	ND
kcat/ Km (μM-1 s-1)	0.082 ± 0.012	0.027 ± 0.003	0.008 ± 0.001	ND

ND, not detectable; Data are shown as mean of three independent experiments (n = 3) ± SE